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MetaMorph Inc
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QImaging
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Qioptiq LINOS
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Image Search Results
Journal: Febs Letters
Article Title: Protein oligomerization mediated by the transmembrane carboxyl terminal domain of Bcl-XL
doi: 10.1016/j.febslet.2011.08.012
Figure Lengend Snippet: Subcellular localization of mRFP and Bcl-XL chimeras. (A) HeLa cells were grown on round coverslips, transfected with vectors encoding myc-mRFP-TMD Bcl-XL, myc-mRFP-TMD VAMP2 or myc-mRFP, incubated with Mitotracker green, fixed with formaldehyde and mounted for microscopy. (B) HeLa cells grown as in A and transfected with myc-BclXL, myc-BclXL-ΔTM, myc-BclXL-TMD2Pit or myc-BclXL-TMD3Pit, incubated with Mitotracker red CMX-Ros, with an anti-myc antibody, with goat anti-mouse Alexa fluor 488-conjugated antibody and mounted for microscopy. Images were acquired with a Leica DMI6000B inverted fluorescence microscope using structured illumination (Optigrid) with filters suitable for mRFP/Mitotracker Red and for Mitotracker green/Alexa fluor 488, and overlayed to show co-localization using software Metamorph. Bar is 10 μm.
Article Snippet: We used a Leica DMI6000B inverted fluorescence microscope with structured
Techniques: Transfection, Incubation, Microscopy, Fluorescence, Software